[The transformation of betA gene into the pollen plantlets of Populus simonii x P. nigra].
Identifieur interne : 003C74 ( Main/Exploration ); précédent : 003C73; suivant : 003C75[The transformation of betA gene into the pollen plantlets of Populus simonii x P. nigra].
Auteurs : Gui-Feng Liu [République populaire de Chine] ; Gui-Lan Cheng ; Jing Jiang ; Shuang Bai ; Ying Yu ; Zhi-Jun Cai ; Jing-Xiang Dong ; Shu-Juan LiSource :
- Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology [ 1671-3877 ] ; 2006.
Descripteurs français
- KwdFr :
- Bétaïne (métabolisme), Chlorure de sodium (pharmacologie), Choline dehydrogenase (génétique), Choline dehydrogenase (métabolisme), Pollen (cytologie), Pollen (génétique), Populus (effets des médicaments et des substances chimiques), Populus (génétique), Populus (métabolisme), RT-PCR (MeSH), Rhizobium (génétique), Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques), Transformation génétique (MeSH), Végétaux génétiquement modifiés (effets des médicaments et des substances chimiques), Végétaux génétiquement modifiés (génétique), Végétaux génétiquement modifiés (métabolisme).
- MESH :
- cytologie : Pollen.
- effets des médicaments et des substances chimiques : Populus, Régulation de l'expression des gènes végétaux, Végétaux génétiquement modifiés.
- génétique : Choline dehydrogenase, Pollen, Populus, Rhizobium, Végétaux génétiquement modifiés.
- métabolisme : Bétaïne, Choline dehydrogenase, Populus, Végétaux génétiquement modifiés.
- pharmacologie : Chlorure de sodium.
- RT-PCR, Transformation génétique.
English descriptors
- KwdEn :
- Betaine (metabolism), Choline Dehydrogenase (genetics), Choline Dehydrogenase (metabolism), Gene Expression Regulation, Plant (drug effects), Plants, Genetically Modified (drug effects), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), Pollen (cytology), Pollen (genetics), Populus (drug effects), Populus (genetics), Populus (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH), Rhizobium (genetics), Sodium Chloride (pharmacology), Transformation, Genetic (MeSH).
- MESH :
- chemical , genetics : Choline Dehydrogenase.
- chemical , metabolism : Betaine, Choline Dehydrogenase.
- cytology : Pollen.
- drug effects : Gene Expression Regulation, Plant, Plants, Genetically Modified, Populus.
- genetics : Plants, Genetically Modified, Pollen, Populus, Rhizobium.
- metabolism : Plants, Genetically Modified, Populus.
- chemical , pharmacology : Sodium Chloride.
- Reverse Transcriptase Polymerase Chain Reaction, Transformation, Genetic.
Abstract
In this study, betA gene was introduced into the pollen plantlets of Populus simonii x P. nigra using Agrobacterium-mediated transformation. The four kanamycine-resistant plants obtained were identified as transgenic plants by PCR detection and the results were all positive. The result of quantitative real-time PCR detection showed that the betA gene was transcribed and expressed in all the transformed plants, but the transcript levels are different. Test of salt-tolerance of the transgenic plants showed that 80%-00% of transgenic plants were rooted while 0 of non-transgenic plants were rooted at 0.55% NaCl stress, and 0 of transgenic plants were rooted at 0.70%-0.80% NaCl stress. The betaine content analysis showed the betaine content of the transgenic plants are obviously higher than that in non-transgenic plants, so transformation betA gene raised the salt tolerance to the transgenic plants.
PubMed: 16622314
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Forestry University, Harbin 150040</wicri:regionArea><wicri:noRegion>Harbin 150040</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Gui Lan" sort="Cheng, Gui Lan" uniqKey="Cheng G" first="Gui-Lan" last="Cheng">Gui-Lan Cheng</name></author><author><name sortKey="Jiang, Jing" sort="Jiang, Jing" uniqKey="Jiang J" first="Jing" last="Jiang">Jing Jiang</name></author><author><name sortKey="Bai, Shuang" sort="Bai, Shuang" uniqKey="Bai S" first="Shuang" last="Bai">Shuang Bai</name></author><author><name sortKey="Yu, Ying" sort="Yu, Ying" uniqKey="Yu Y" first="Ying" last="Yu">Ying Yu</name></author><author><name sortKey="Cai, Zhi Jun" sort="Cai, Zhi Jun" uniqKey="Cai Z" first="Zhi-Jun" last="Cai">Zhi-Jun Cai</name></author><author><name sortKey="Dong, Jing Xiang" sort="Dong, Jing Xiang" uniqKey="Dong J" first="Jing-Xiang" last="Dong">Jing-Xiang Dong</name></author><author><name sortKey="Li, Shu Juan" sort="Li, Shu Juan" uniqKey="Li S" first="Shu-Juan" last="Li">Shu-Juan Li</name></author></analytic><series><title level="j">Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology</title><idno type="ISSN">1671-3877</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Betaine (metabolism)</term><term>Choline Dehydrogenase (genetics)</term><term>Choline Dehydrogenase (metabolism)</term><term>Gene Expression Regulation, Plant (drug effects)</term><term>Plants, Genetically Modified (drug effects)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>Pollen (cytology)</term><term>Pollen (genetics)</term><term>Populus (drug effects)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term><term>Rhizobium (genetics)</term><term>Sodium Chloride (pharmacology)</term><term>Transformation, Genetic (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Bétaïne (métabolisme)</term><term>Chlorure de sodium (pharmacologie)</term><term>Choline dehydrogenase (génétique)</term><term>Choline dehydrogenase (métabolisme)</term><term>Pollen (cytologie)</term><term>Pollen (génétique)</term><term>Populus (effets des médicaments et des substances chimiques)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>RT-PCR (MeSH)</term><term>Rhizobium (génétique)</term><term>Régulation de l'expression des gènes végétaux (effets des médicaments et des substances chimiques)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (effets des médicaments et des substances chimiques)</term><term>Végétaux génétiquement modifiés (génétique)</term><term>Végétaux génétiquement modifiés (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Choline Dehydrogenase</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Betaine</term><term>Choline Dehydrogenase</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Pollen</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Plants, Genetically Modified</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Populus</term><term>Régulation de l'expression des gènes végétaux</term><term>Végétaux génétiquement modifiés</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants, Genetically Modified</term><term>Pollen</term><term>Populus</term><term>Rhizobium</term></keywords><keywords scheme="MESH" 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génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study, betA gene was introduced into the pollen plantlets of Populus simonii x P. nigra using Agrobacterium-mediated transformation. The four kanamycine-resistant plants obtained were identified as transgenic plants by PCR detection and the results were all positive. The result of quantitative real-time PCR detection showed that the betA gene was transcribed and expressed in all the transformed plants, but the transcript levels are different. Test of salt-tolerance of the transgenic plants showed that 80%-00% of transgenic plants were rooted while 0 of non-transgenic plants were rooted at 0.55% NaCl stress, and 0 of transgenic plants were rooted at 0.70%-0.80% NaCl stress. The betaine content analysis showed the betaine content of the transgenic plants are obviously higher than that in non-transgenic plants, so transformation betA gene raised the salt tolerance to the transgenic plants.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16622314</PMID><DateCompleted><Year>2009</Year><Month>03</Month><Day>19</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1671-3877</ISSN><JournalIssue CitedMedium="Print"><Volume>32</Volume><Issue>2</Issue><PubDate><Year>2006</Year><Month>Apr</Month></PubDate></JournalIssue><Title>Zhi wu sheng li yu fen zi sheng wu xue xue bao = Journal of plant physiology and molecular biology</Title><ISOAbbreviation>Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[The transformation of betA gene into the pollen plantlets of Populus simonii x P. nigra].</ArticleTitle><Pagination><MedlinePgn>163-8</MedlinePgn></Pagination><Abstract><AbstractText>In this study, betA gene was introduced into the pollen plantlets of Populus simonii x P. nigra using Agrobacterium-mediated transformation. The four kanamycine-resistant plants obtained were identified as transgenic plants by PCR detection and the results were all positive. The result of quantitative real-time PCR detection showed that the betA gene was transcribed and expressed in all the transformed plants, but the transcript levels are different. Test of salt-tolerance of the transgenic plants showed that 80%-00% of transgenic plants were rooted while 0 of non-transgenic plants were rooted at 0.55% NaCl stress, and 0 of transgenic plants were rooted at 0.70%-0.80% NaCl stress. The betaine content analysis showed the betaine content of the transgenic plants are obviously higher than that in non-transgenic plants, so transformation betA gene raised the salt tolerance to the transgenic plants.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Gui-Feng</ForeName><Initials>GF</Initials><AffiliationInfo><Affiliation>Heilongjiang Provincial Key Laboratory of Teaching and Research Section of Forestry Genetics and Breeding, Northeast Forestry University, Harbin 150040, China. liuguifeng@126.com</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Gui-Lan</ForeName><Initials>GL</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Jing</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Bai</LastName><ForeName>Shuang</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Ying</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Zhi-Jun</ForeName><Initials>ZJ</Initials></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Jing-Xiang</ForeName><Initials>JX</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Shu-Juan</ForeName><Initials>SJ</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao</MedlineTA><NlmUniqueID>101156321</NlmUniqueID><ISSNLinking>1671-3877</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>3SCV180C9W</RegistryNumber><NameOfSubstance UI="D001622">Betaine</NameOfSubstance></Chemical><Chemical><RegistryNumber>451W47IQ8X</RegistryNumber><NameOfSubstance UI="D012965">Sodium Chloride</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.1.99.1</RegistryNumber><NameOfSubstance UI="D050545">Choline Dehydrogenase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001622" MajorTopicYN="N">Betaine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D050545" MajorTopicYN="N">Choline Dehydrogenase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011058" MajorTopicYN="N">Pollen</DescriptorName><QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012231" MajorTopicYN="N">Rhizobium</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012965" MajorTopicYN="N">Sodium Chloride</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>4</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>3</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>4</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16622314</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Bai, Shuang" sort="Bai, Shuang" uniqKey="Bai S" first="Shuang" last="Bai">Shuang Bai</name><name sortKey="Cai, Zhi Jun" sort="Cai, Zhi Jun" uniqKey="Cai Z" first="Zhi-Jun" last="Cai">Zhi-Jun Cai</name><name sortKey="Cheng, Gui Lan" sort="Cheng, Gui Lan" uniqKey="Cheng G" first="Gui-Lan" last="Cheng">Gui-Lan Cheng</name><name sortKey="Dong, Jing Xiang" sort="Dong, Jing Xiang" uniqKey="Dong J" first="Jing-Xiang" last="Dong">Jing-Xiang Dong</name><name sortKey="Jiang, Jing" sort="Jiang, Jing" uniqKey="Jiang J" first="Jing" last="Jiang">Jing Jiang</name><name sortKey="Li, Shu Juan" sort="Li, Shu Juan" uniqKey="Li S" first="Shu-Juan" last="Li">Shu-Juan Li</name><name sortKey="Yu, Ying" sort="Yu, Ying" uniqKey="Yu Y" first="Ying" last="Yu">Ying Yu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Liu, Gui Feng" sort="Liu, Gui Feng" uniqKey="Liu G" first="Gui-Feng" last="Liu">Gui-Feng Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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